金黄色葡萄球菌对体外保存猪精子活力及蛋白磷酸化修饰的影响

doi:10.11843/j.issn.0366-6964.2017.09.011

Abstract

Abstract:

This study aimed to investigate the effects of S. aureus on boar sperm motility during storage at 17℃ from the perspectives of sperm metabolism and protein phosphorylation modification levels, and provide a theoretical basis for the molecular mechanisms underlying the study of pathogens on sperm quality. Sperm motility parameters were determined using computer assisted sperm analysis (CASA) and the varying of intracellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity, cyclic adenosine monophosphate (cAMP) content and totally ATP level were measured by reagent kits. Protein phosphorylation levels and the localization of phosphorylated targets were analyzed by Western blot and immunofluorescence technique, respectively. The results of CASA showed that 10³ CFU·mL^-1 S. aureus-treated could significantly inhibit sperm motility during boar semen storage at 17℃ (P<0.05). With the increase of S.aureus, sperm motility parameters were significantly decreased after 3 days of inoculation (P<0.01). Similarly, GAPDH activity and ATP level in the treatment groups were also markedly decreased compared to that in the control groups after 3 days of inoculation (P<0.05). The results of Western blot showed that there were significant differences in sperm protein phosphorylation levels between the treatment groups and the control groups (P<0.05). The changing trends of phosphorylation levels were consistent with that of intracellular cAMP level, which suggested that S.aureus affecting protein phosphorylation levels might following the sAC-cAMP-PKA signaling pathway. Taken together, S.aureus reduced intracellular ATP content through lowering glycolytic GAPDH activity, meanwhile, S.aureus inhibited the phosphorylation levels of motility-related proteins located in the posterior nucleus, equatorial piece and flagellar regions of boar sperm following the sAC-cAMP-PKA signaling pathway. Both the reduction of ATP and the inhibition of protein phosphorylation levels led to the decrease of boar sperm motility.

CLC Number:

S828.2

LI Yu-hua, FU Jie-li, LI Pei-fei, YANG Qiang-zhen, WANG Li-rui, XIE Wei-yi, LI Xin-hong. Effects of Staphylococcus aureus on Motility and Protein Phosphorylation Modification of Boar Sperm during Storage in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1665-1673.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2017.09.011

https://www.xmsyxb.com/EN/Y2017/V48/I9/1665

References

[1] ALTHOUSE G C, LU K G. Bacteriospermia in extended porcine semen[J]. Theriogenology, 2005, 63(2):573-584.
[2] ALTHOUSE G C, KUSTER C E, CLARK S G, et al. Field investigations of bacterial contaminants and their effects on extended porcine semen[J]. Theriogenology, 2000, 53(5):1167-1176.
[3] MAROTO MARTÍN L O, MUÑOZ E C, DE CUPERE F, et al. Bacterial contamination of boar semen affects the litter size[J]. Anim Reprod Sci, 2010, 120(1-4):95-104.
[4] SEPÚLVEDA L, BUSSALLEU E, YESTE M, et al. Effects of different concentrations of Pseudomonas aeruginosa on boar sperm quality[J]. Anim Reprod Sci, 2014, 150(3-4):96-106.
[5] DONG H T, SHI W S, TIAN Y, et al. Expression and tyrosine phosphorylation of sp32 regulate the activation of the boar proacrosin/acrosin system[J]. Genet Mol Res, 2015, 14(1):2374-2383.
[6] SEPÚLVEDA L, BUSSALLEU E, YESTE M, et al. Effect of Pseudomonas aeruginosa on sperm capacitation and protein phosphorylation of boar spermatozoa[J]. Theriogenology, 2016, 85(8):1421-1431.
[7] PRIETO-MARTÍNEZ N, BUSSALLEU E, GARCIA-BONAVILA E, et al. Effects of Enterobacter cloacae on boar sperm quality during liquid storage at 17℃[J]. Anim Reprod Sci, 2014, 148(1-2):72-82.
[8] SEPÚLVEDA L, BUSSALLEU E, YESTE M, et al. How do different concentrations of Clostridium perfringens affect the quality of extended boar spermatozoa?[J]. Anim Reprod Sci, 2013, 140(1-2):83-91.
[9] BUSSALLEU E, YESTE M, SEPU'LVEDA L, et al. Effects of different concentrations of enterotoxigenic and verotoxigenic E. coli on boar sperm quality[J]. Anim Reprod Sci, 2011, 127(3-4):176-182.
[10] FRACZEK M, HRYHOROWICZ M, GACZARZEWICZ D, et al. Can apoptosis and necrosis coexist in ejaculated human spermatozoa during in vitro semen bacterial infection?[J]. J Assist Reprod Genet, 2015, 32(5):771-779.
[11] DIEMER T, HUWE P, MICHELMANN H W, et al. Escherichia coli-induced alterations of human spermatozoa. An electron microscopy analysis[J]. Int J Androl, 2000, 23(3):178-186.
[12] WOLFF H, PANHANS A, STOLZ W, et al. Adherence of Escherichia coli to sperm:a mannose mediated phenomenon leading to agglutination of sperm and E. coli[J]. Fertil Steril, 1993, 60(1):154-158.
[13] QIANG H, JIANG M S, LIN J Y, et al. Influence of enterococci on human sperm membrane in vitro[J]. Asian J Androl, 2007, 9(1):77-81.
[14] FRACZEK M, SZUMALA-KAKOL A, JEDRZEJCZAK P, et al. Bacteria trigger oxygen radical release and sperm lipid peroxidation in in vitro model of semen inflammation[J]. Fertil Steril, 2007, 88(Suppl 4):1076-1085.
[15] DU PLESSIS S S, AGARWAL A, MOHANTY G, et al. Oxidative phosphorylation versus glycolysis:what fuel do spermatozoa use?[J]. Asian J Androl, 2015, 17(2):230-235.
[16] JHA K N, SHIVAJI S. Protein serine and threonine phosphorylation, hyperactivation and acrosome reaction in in vitro capacitated hamster spermatozoa[J]. Mol Reprod Dev, 2002, 63(1):119-130.
[17] ZHEN L Q, WANG L R, FU J L, et al. Hexavalent chromium affects sperm motility by influencing protein tyrosine phosphorylation in the midpiece of boar spermatozoa[J]. Reprod Toxicol, 2016, 59:66-79.
[18] KUSTER C E, ALTHOUSE G C. The impact of bacteriospermia on boar sperm storage and reproductive performance[J]. Theriogenology, 2016, 85(1):21-26.
[19] MUKAI C, OKUNO M. Glycolysis plays a major role for adenosine triphosphate supplementation in mouse sperm flagellar movement[J]. Biol Reprod, 2004, 71(2):540-547.
[20] MIKI K, QU W D, GOULDING E H, et al. Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility[J]. Proc Natl Acad Sci U S A, 2004, 101(47):16501-16506.
[21] OH S A, PARK Y J, YOU Y A, et al. Capacitation status of stored boar spermatozoa is related to litter size of sows[J]. Anim Reprod Sci, 2010, 121(1-2):131-138.
[22] MATÁS C, SANSEGUNDO M, RUIZ S, et al. Sperm treatment affects capacitation parameters and penetration ability of ejaculated and epididymal boar spermatozoa[J]. Theriogenology, 2010, 74(8):1327-1340.
[23] URNER F, SAKKAS D. Protein phosphorylation in mammalian spermatozoa[J]. Reproduction, 2003, 125(1):17-26.
[24] VISCONTI P E. Understanding the molecular basis of sperm capacitation through kinase design[J]. Proc Natl Acad Sci U S A, 2009, 106(3):667-668.

Effects of Staphylococcus aureus on Motility and Protein Phosphorylation Modification of Boar Sperm during Storage in vitro

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	NIU Naiqi, ZHAO Runze, ZONG Wencheng, LIU Xiance, LIU Hai, SHI Guohua, JING Xitao, ZHANG Longchao. Association of Polymorphisms of GREB1L and MIB1 Genes with Rib Number and Carcass Traits in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 79-86.
[2]	ZHU Xueli, ZHANG Longchao, WANG Lixian, PU Lei, LIU Xin. Association Analysis of AQP9 and RPS10 Gene Polymorphisms with Backfat Thickness in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 87-98.
[3]	YANG Kai, LU Zhuoda, HE Jian, ZHANG Ruiqi, WANG Suqing, LI Kebiao, ZHAO Yunxiang, ZHU Xiaoping, GUO Jinbiao. The Population Effect of Commercial Pigs on the Accuracy of Genomic Selection for Carcass Traits in Duroc Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4943-4951.
[4]	XU Tingting, QI Fenfang, HUANG Shihui, NIU Xi, LI Sheng, RAN Xueqin, WANG Jiafu, XIE Jian. Study of the Polymorphisms of Structural Variation and the Expression of MAP3K4 gene in Xiang Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5046-5055.
[5]	HU Ziping, WANG Ligang, ZONG Wencheng, HOU Renda, SU Yanfang, NIU Naiqi, WANG Lixian, WANG Yuan, ZHNAG Longchao. Genetic Structure Analysis of Jianbai Xiang Pig Population Based on Genomic SNP and ROH [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4117-4125.
[6]	JI Zhengyu, NI Mengru, ZHANG Zhaobo, ZHAO Gan, HUANG Zan, LI Pinghua, HUANG Ruihua, HOU Liming. Research on Adipogenic Capacity and Gene Expression Pattern of in Vitro FAPs Cells Derived from Longissimus Dorsi Muscle of Suhuai Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4126-4142.
[7]	BI Huan, QIN Hai, YUAN Wei, ZHANG Yudan, ZHANG Yiyu, CHEN Wei. Effect of Knocking down TYRP1 Gene on Melanin Production in the Xiang Pig Epidermal Melanocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4143-4153.
[8]	WANG Hui, FENG Baoliang, WU Dan, XIANG Guangming, WANG Nan, MU Yulian, LI Kui, LIU Zhiguo. Research Progress of CD163 Gene and Disease-Resistant Breeding on Porcine Reproductive and Respiratory Syndrome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3127-3138.
[9]	ZHANG Wanfeng, ZHAO Tianzhi, LI Jiao, YOU Ziwei, YANG Yang, CAI Chunbo, GAO Pengfei, CAO Guoqing, GUO Xiaohong, LI Bugao. Study on NR2F2 Gene Regulating Proliferation and Apoptosis of Porcine PK15 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3242-3251.
[10]	LU Chang, DONG Lei, ZHANG Wanfeng, GAO Pengfei, GUO Xiaohong, CAI Chunbo, CAO Guoqing, LI Bugao. Identification and Screening of Single Nucleotide Polymorphism Loci in Jinfen White Pigs Based on Whole Genome Resequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2761-2771.
[11]	YANG Qing, GONG Jing, ZHAO Xueyan, ZHU Xiaodong, GENG Liying, ZHANG Chuansheng, WANG Jiying. Comparison of Array and Resequencing in Pig Genetic Structure Studies [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2772-2782.
[12]	WANG Jinglin, LIU Yangguang, XU Qilong, CHEN Shuo, DENG Zaishuang, CHENG Shiyu, DING Yueyun, ZHENG Xianrui, YIN Zongjun, ZHANG Xiaodong. Genome Structures Variant Analysis and Feature SNPs Screening of Wanyue Black Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2783-2793.
[13]	ZHOU Fuchen, YE Jian, GUO Caijin, ZENG Haiyu, WU Zhenfang, DONG Linsong, CAI Gengyuan. Litter Effect Analysis and Genetic Parameters Estimation of Lean Meat Percentage Trait in Duroc Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2288-2296.
[14]	ZHAO Zhenjian, WANG Shujie, CHEN Dong, JI Xiang, SHEN Qi, YU Yang, CUI Shengdi, WANG Junge, CHEN Ziyang, TANG Guoqing. Population Structure and Genetic Diversity Analysis of Neijiang Pigs Based on Low-coverage Whole Genome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2297-2307.
[15]	TAO Xuan, YANG Xuemei, LIANG Yan, LIU Yihui, WANG Yong, KONG Fanjing, LEI Yunfeng, YANG Yuekui, WANG Yan, AN Rui, YANG Kun, Lü Xuebin, HE Zhiping, GU Yiren. Analysis of Genetic Structure of Conservation Population in Yacha Pig Based on SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2308-2319.